2009 Vol.13 (2): 52-54 [PDF全文]
【文章题目】卷丹百合RAPD-PCR反应程序的优化研究
【作者】韩 凌；雷家军
【关键词】卷丹百合；RAPD；正交设计；单因素试验；程序优化
【摘要】采用CTAB法提取卷丹百合的基因组DNA。采用正交设计和单因素试验相结合的方法，对卷丹百合RAPD-PCR反应程序中各影响因素进行了优化。结果表明：卷丹百合最佳的RAPD-PCR扩增程序为94 ℃预变性4 min；94 ℃变性30 s、39 ℃退火40 s、72 ℃延伸1 min，45个循环；72 ℃延伸10 min。
【基金项目】
【Title】 Optimization of RAPD-PCR Procedure in Lilium lancifolium Thunb.
【Authors】 HAN Ling，LEI Jia-jun
【Key words】 Lilium lancifolium Thunb.；RAPD.；Orthogonal design；Single factor design；Procedure optimization
【Abstract】 Genomic DNA of [WTBX]Lilium lancifolium.Thunbwas extracted with the method of CTABSome influencing factors in the RAPD reaction procedure were studied with orthogonal design and single factor experimentThe results showed that the optimum RAPD-PCR procedure was one cycle of denaturing at 94 ℃ for 4 min，45 cycles of denaturing at 94 ℃ for 30 s，annealing at 39 ℃ for 40 s，extending at 72 ℃ for 1 min，one cycle of final extension extending at 72 ℃ for 10 min.